How to Safely Reconstitute Peptides (Research Use Only)

Reconstituting peptides correctly is essential for research experiments. Proper reconstitution ensures accurate concentrations and preserves peptide stability.

⚠️ For research use only. Not for human or animal use.

Step 1: Select the Right Solvent
Peptides can be water-soluble, alcohol-soluble, or require acidic/basic buffers. Use only the solvent recommended by the manufacturer for laboratory research.

Step 2: Calculate Solvent Volume
Determine the final concentration you need. Use the formula: Concentration (mg/mL) = Peptide Mass ÷ Solvent Volume. Refer to our previous blog post for mathematical references.

Step 3: Add Solvent Carefully
Use sterile pipettes or syringes. Gently add solvent to the peptide vial — avoid vigorous shaking as some peptides are sensitive to shear forces.

Step 4: Mix and Label
Invert the vial gently until the peptide is fully dissolved. Label the vial with the peptide name, concentration, and date.

Step 5: Storage
Store reconstituted peptides at the recommended temperature, usually refrigerated or frozen for longer storage. Avoid repeated freeze-thaw cycles.

Step 6: Safety Reminder
Always follow laboratory safety protocols. These guidelines are intended for research use only and not for human or animal administration.