Sterile Technique 101: Keeping Your Workspace Clean and Safe

Why Sterile Technique Matters

Contamination can compromise materials, distort experimental results, and increase the risk of accidental exposure. Sterile technique reduces these risks by keeping equipment and surfaces clean while preventing the introduction of unwanted particles or microbes.

1. Start With a Clean Workspace

The issue: Dust, residues, and uncleaned surfaces increase chances of contamination.

Best practice: Before setting up, wipe the workspace with an appropriate disinfectant or alcohol-based surface cleaner. Allow surfaces to dry fully before beginning.

2. Wash and Glove Properly

The issue: Hands transfer oils, particles, and contaminants even if they appear clean.

Best practice: Wash hands thoroughly and put on fresh gloves. Change gloves if they tear, become visibly soiled, or contact non-sterile surfaces.

3. Keep Tools Sterile

The issue: Using tools that have not been cleaned or stored properly can introduce contaminants.

Best practice: Use sterile or freshly cleaned equipment. For disposable tools, use a new item for each critical operation. Avoid placing tools on non-sterile surfaces.

4. Minimize Airflow and Movement

The issue: Air currents carry dust and particles that can land on open containers or equipment.

Best practice: Work in an area with minimal airflow. Keep container lids closed when not in use. Avoid unnecessary talking, movement, or reaching over open materials.

5. Use Proper Technique When Opening Vials

The issue: Touching vial tops, stoppers, or septa can transfer contaminants.

Best practice: Swab vial tops with alcohol before use. Allow them to dry. When inserting needles, avoid touching the needle or the septum after cleaning.

6. Dispose of Waste Safely

The issue: Allowing used needles, swabs, or containers to accumulate increases safety risks.

Best practice: Immediately discard sharps into a designated sharps container. Dispose of wipes, packaging, and gloves in the appropriate waste bin when finished.

Final Tips

Sterile technique is as much about habit as it is about equipment. Consistency makes workflows smoother and results more reliable.